Before the ultrasonic pulverizer is used, it is necessary to insert the ultrasonic transformer into the sample before it can be turned on. No no-load operation can be carried out.
Ultrasonic grinding device of ultrasonic parameters Settings: set the key instrument working parameters, is more sensitive to the requirement of temperature of the sample outside commonly use an ice bath, actual temperature must be lower than 25 degrees, protein, nucleic acid will surely not degeneration.
1. Time: the ultrasonic time should not be more than 5 seconds each time, the clearance time should be greater than or equal to the ultrasonic time, so as to be distributed in the heat. The time setting should be short ultrasonic time and multi-principle of ultrasonic frequency, which can prolong the life of ultrasonic machine and probe.
2, ultrasonic power: shoulds not be too big, lest sample splash or foaming, such as less than 10 ml sample capacity, power should be within the 200 w, choose 2 mm ultrasonic probe, the other will be at the back of the panel and discussed.the selection switch to the corresponding block; 10-200ml sample capacity of 2000-400w, 6mm ultrasonic probe was selected, and the variation bar behind the panel was hit to the corresponding block; The sample capacity of more than 200ml is between 300-600w, and 10mm ultrasonic probe is selected, and the variation bar behind the panel is hit to the corresponding block.
3. The container of ultrasonic pulverizer: how many samples should be selected for the size of the beater, so that the favorable sample can be convection in the ultrasonic and improve the crushing efficiency.
4. In order to prevent the drop of liquid level after the melting of ice bath, the sample needs to be placed in a 1.5ml EP tube to keep the EP tube fixed.
5. The depth of the ultrasonic probe (variation rod) is about 1.5cm, and the liquid level is above 30mm. The probe should be centered, and no wall can be adhered to. Ultrasonic wave is a vertical longitudinal wave, and the insertion is too deep to form convection, which can directly affect the crushing efficiency.