The principle of ultrasonic cell crusher is not too mysterious, too complex. Simply put, the energy through the transducer converted to sound energy, which through the liquid medium to become a dense small bubble, these small bubbles quickly burst, resulting in the same energy as small bombs, thus playing the role of broken cells and other substances.
Ultrasonic is a kind of elastic mechanical wave in material medium, it is a kind of wave form, so it can be used to detect physiological and pathological information of human body, not only diagnose ultrasound. At the same time, it is an energy form, when a certain dose of ultrasound in vivo transmission, through their interaction, can cause the function and structure of the organism changes, namely the ultrasonic biological effect. The effects of ultrasound on cells mainly include heat effect, cavitation effect and mechanical effect. The heat effect is when the ultrasound is propagating in the medium, friction hinders the molecular vibration caused by ultrasound, which converts some energy into local high fever (42-43 ℃), because the critical lethal temperature of normal tissue is 45.7 ℃, and the tumor tissue is more sensitive than normal tissue, so the metabolic disorder of tumor cells occurs at this temperature, DNA, RNA, protein synthesis is affected, thus killing cancer cells and normal tissue is unaffected. Cavitation effect is the formation of cavitation in the body under the ultrasonic irradiation, and the mechanical shear pressure and turbulence resulting from the shock of cavitation and its violent coalescence result in the necrosis of the tumor bleeding and tissue disintegration. In addition, cavitation bubble rupture produces instantaneous high temperature (about 5,000 ℃), high pressure (up to 500X104PA), can make steam pyrolysis away from production. Oh free radicals and. h atoms, redox reactions caused by. Oh free radicals and. h atoms can lead to poly degradation, enzyme inactivation, lipid peroxidation and cell killing. Mechanical effect is the primary effect of ultrasound, in the process of the propagation of the medium particles in the alternating compression and the extension of pressure changes, resulting in cell structure damage. The strength of killing is closely related to the frequency and intensity of ultrasound.